A single-page web front-end for vflank — paste, upload, or type a handful of variants and get back masked ddPCR target sequences (and optional Primer3 input), with no install and no patient data leaving the browser.
- Editable grid (type · paste-from-Excel · upload-populates) with live, vflank-authoritative validation before you run.
- No-download: reference comes from the UCSC API and population frequencies from gnomAD — there is no BAM and no PHI in this tool (modes A/B only).
- A thin FastAPI service over
vflank.run_small/run_fusion; all the science lives in vflank (this repo is UI + hosting policy).
Scope: single variant / tiny batch (≤10 records). For bulk, fusions with BAM consensus, or offline use, use the vflank CLI / PyPI package.
| Small variant | Fusion |
|---|---|
 |
 |
Paste a variant, press Run, and get the masked flanking sequence (small variant) or chimeric junction (fusion) to download. Full narrated walkthroughs are on the vflank docs — Web tool page.
pip install -e ".[dev]"
uvicorn app.main:app --reload
# open http://127.0.0.1:8000
The button above provisions a free Render web service from the render.yaml
blueprint (Python runtime: pip install . → uvicorn app.main:app, health
check /healthz). After the initial setup, pushes to main redeploy
automatically. A Dockerfile is also provided for container parity / other hosts.
- Usage — how to use the UI.
- Architecture — how the service works (and what it delegates to vflank).
- Developer guide — setup, the gate, git-flow, deploy.
For the science (masking modes, gnomAD AF semantics, coordinate conventions, the
run_small API), see the vflank docs —
this repo does not duplicate them.
Apache-2.0, matching vflank.