Skip to content

Releases: obenno/scRNA-seq

v1.2.9

27 Nov 05:08

Choose a tag to compare

  • Updated docker image building workflow

v1.1.8

07 Oct 07:09

Choose a tag to compare

  • Updated VDJ filtration criteria when only BCR/TCR library was used
  • VDJ library barcodes will be filtered by cells identified in GEX library
  • Minor bug fixed for VDJ pipeline
  • Constrained fastqc>=0.11.9 cutadapt>=3.5in conda yml file
  • VDJ pipeline will publish trust4 report and airr file now
  • Fixed barcodelist bug when invoking complex barcode pipeline for BD style barcode

v1.1.6

13 May 11:57

Choose a tag to compare

  • Updated saturation script to include data as preseqR input
  • Updated VDJ subworkflow to adapt to the new library structure
  • Fix the feature_types bug for VDJ report process
  • Added GEX association section to the VDJ report

v1.1.3

23 Dec 08:17

Choose a tag to compare

Added --outBAMsortingBinsN 300 option to STARsolo process to solve the RAM issue when sorting a very large BAM file, refer to STAR #870

v1.1.2

09 Nov 08:05

Choose a tag to compare

updated to v1.1.2

v1.1.1

17 Oct 14:12

Choose a tag to compare

  • Added memory setup in the report process
  • Added option for publishing starsolo BAM output

v1.1.0 VDJ pipeline added

14 Sep 02:53

Choose a tag to compare

  • Added VDJ workflow (GEX+VDJ)
  • Removed time limit for process resources
  • Modified the margin of the tables in the 3'-scRNA-Seq report
  • Adjusted knee plot to fixed size and center positioned

v1.0.0

10 Aug 01:55

Choose a tag to compare

  • Added pipeline running information to the reports
  • Fixed issue for saturation calculation script when there is no whitelist provided
  • Output a full list of DEGs

v0.0.9

08 Aug 06:08

Choose a tag to compare

  • Updated STAR to v2.7.10a in both the docker image and the conda environment
  • Adapt the pipeline to generate report for starsolo --soloMultiMappers option, only "Unique" and "EM" are supported by now.
  • Changed the default parameter for --soloFeatures to "GeneFull", which includes both exon and intron reads

v0.0.7

15 Jul 11:46

Choose a tag to compare

  • Raw gene/barcode count table will be stored in a loom file and published for subsequent analysis
  • Mapping metrics will be written to a json file
  • Added support for 5' RNA-seq library