Releases: obenno/scRNA-seq
Releases · obenno/scRNA-seq
v1.2.9
27 Nov 05:08
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Updated docker image building workflow
v1.1.8
07 Oct 07:09
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Updated VDJ filtration criteria when only BCR/TCR library was used
VDJ library barcodes will be filtered by cells identified in GEX library
Minor bug fixed for VDJ pipeline
Constrained fastqc>=0.11.9 cutadapt>=3.5in conda yml file
VDJ pipeline will publish trust4 report and airr file now
Fixed barcodelist bug when invoking complex barcode pipeline for BD style barcode
v1.1.6
13 May 11:57
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Updated saturation script to include data as preseqR input
Updated VDJ subworkflow to adapt to the new library structure
Fix the feature_types bug for VDJ report process
Added GEX association section to the VDJ report
v1.1.3
23 Dec 08:17
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Added --outBAMsortingBinsN 300 option to STARsolo process to solve the RAM issue when sorting a very large BAM file, refer to STAR #870
v1.1.2
09 Nov 08:05
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v1.1.1
17 Oct 14:12
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Added memory setup in the report process
Added option for publishing starsolo BAM output
v1.1.0 VDJ pipeline added
14 Sep 02:53
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Added VDJ workflow (GEX+VDJ)
Removed time limit for process resources
Modified the margin of the tables in the 3'-scRNA-Seq report
Adjusted knee plot to fixed size and center positioned
v1.0.0
10 Aug 01:55
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Added pipeline running information to the reports
Fixed issue for saturation calculation script when there is no whitelist provided
Output a full list of DEGs
v0.0.9
08 Aug 06:08
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Updated STAR to v2.7.10a in both the docker image and the conda environment
Adapt the pipeline to generate report for starsolo --soloMultiMappers option, only "Unique" and "EM" are supported by now.
Changed the default parameter for --soloFeatures to "GeneFull", which includes both exon and intron reads
v0.0.7
15 Jul 11:46
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Raw gene/barcode count table will be stored in a loom file and published for subsequent analysis
Mapping metrics will be written to a json file
Added support for 5' RNA-seq library