This repository contains an ongoing computational transcriptomics project investigating the molecular effects of EZH2 inhibition in OV8 ovarian cancer cells.The project focuses on identifying transcriptomic alterations associated with epigenetic dysregulation and therapeutic inhibition of EZH2.
EZH2-mediated chromatin remodeling contributes to ovarian cancer progression, proliferation, and therapeutic resistance.Targeting EZH2 has emerged as a promising epigenetic therapeutic strategy in ovarian carcinoma. This project evaluates transcriptomic responses induced by shEZH2 knockdown and DZNep treatment in OV8 ovarian cancer cells.
- Identify differentially expressed genes following EZH2 inhibition
- Investigate pathways associated with proliferation and metabolism
- Evaluate transcriptomic consequences of epigenetic dysregulation
- Explore potential therapeutic mechanisms in ovarian cancer
Cell Line:
- OV8 ovarian cancer cells Experimental Conditions:
- shEZH2 knockdown
- DZNep treatment
- untreated controls Data Source:
- Publicly available GEO/SRA datasets
FASTQC -> HISAT2 (Read Alignment) -> Infer Experiment -> StringTie (Transcript Assembly) -> DESeq2 (Differential Expression Analysis)-> Gene Ontology (GO) and Pathway Enrichment Analysis -> Biological Interpretation
- Galaxy Europe
- HISAT2
- StringTie
- DESeq2
- Excel
- ToppGene
Ongoing Computational Oncology Project
Expected Completion: July 2026
Current Progress:
- Dataset acquisition completed
- FASTQ preprocessing initiated
- Differential expression analysis pending
- EZH2 inhibition
- Ovarian cancer epigenetics
- Chromatin remodeling
- Metabolic pathway alterations
- Therapeutic response mechanisms
- Comparative analysis across additional ovarian cancer datasets
- Integration with pathway-focused therapeutic interpretation
- Translational analysis of epigenetic inhibitor response signatures
Sriprata Ramasubramnian MSc.Medical Genetics & Genomics