Antibiotics

Preparation of stocks

Antibiotic	Color	Abbr	Concentration (mg/ml)	Solvent	Stock notes
Carbenicillin	ORANGE	Carb	100	dH₂0	Filter
Chloramphenicol	RED	Cam	25	100% ethanol
Erythromycin	GREEN	Erm	10	100% ethanol
Kanamycin	BLUE	Kan	50	dH₂0	Filter
Spectinomycin	LIME	Spc	50	dH₂0	Filter

Note that the dry erythromycin is stored at room temperature.

For each stock aliquot approximately 700 μl per microfuge tube and store at -20 °C.

Note that there is additional information about making antibiotic-containing media listed under media.

Antibiotic	Color	Final concentration (μg / ml)	Stock to add per 1 L media
Carbenicillin	ORANGE	LB-Carb¹⁰⁰	1,000 μl
Chloramphenicol	RED	LB-Cam²⁵	1,000 μl
Erythromycin	GREEN	BHI-Erm¹⁵⁰	150 mg dry (or 15 ml)
Kanamycin	BLUE	LB-Kan⁵⁰	1,000 μl
Spectinomycin	LIME	LB-Spc⁵⁰	1,000 μl
Tetracycline	PURPLE	LB-Tet¹⁵	1,500 μl

Antibiotic	Color	Final concentration (μg / ml)	Stock to add per 1 L media
Carbenicillin	ORANGE	N/A
Chloramphenicol	RED	LBS-Cam⁵	200 μl
Erythromycin	GREEN	LBS-Erm⁵	500 μl
Kanamycin	BLUE	LBS-Kan¹⁰⁰	2,000 μl
Tetracycline	PURPLE	LBS-Tet⁵	500 μl