For some long reads (10kbp), if their left ends are nearly aligned, should we directly use SPOA to generate a consensus sequence, or adopt a segmented SPOA approach (e.g., segment the reads into 200bp windows first and then merge the results via SPOA)?
For some long reads (10kbp), if their left ends are nearly aligned, should we directly use SPOA to generate a consensus sequence, or adopt a segmented SPOA approach (e.g., segment the reads into 200bp windows first and then merge the results via SPOA)?