diff --git a/packages/jest-preset/README.md b/packages/jest-preset/README.md
index de420553b828..c53da96056d0 100644
--- a/packages/jest-preset/README.md
+++ b/packages/jest-preset/README.md
@@ -36,9 +36,3 @@ module.exports = {
 ```
 
 You can further customize your Jest configuration by specifying other options. See [Jest's `jest.config.js` documentation](https://jestjs.io/docs/configuration) to learn more.
-
-### Migration Note
-
-This Jest preset used to be part of the core `react-native` package and accessible at `react-native/jest-preset.js`. As long as `@react-native/jest-preset` is installed, `react-native/jest-preset.js` will be aliased to this package and continue to work but is deprecated.
-
-Follow the installation instructions above to migrate to `@react-native/jest-preset` and change `preset: 'react-native'` to `preset: '@react-native/jest-preset` to migrate.
diff --git a/packages/react-native/jest-preset.js b/packages/react-native/jest-preset.js
deleted file mode 100644
index 5251fcdbd0d2..000000000000
--- a/packages/react-native/jest-preset.js
+++ /dev/null
@@ -1,26 +0,0 @@
-/**
- * Copyright (c) Meta Platforms, Inc. and affiliates.
- *
- * This source code is licensed under the MIT license found in the
- * LICENSE file in the root directory of this source tree.
- *
- * @noflow
- * @format
- */
-
-'use strict';
-
-try {
-  module.exports = require('@react-native/jest-preset');
-} catch (error) {
-  if (error.code === 'MODULE_NOT_FOUND') {
-    throw new Error(
-      `The React Native Jest preset has moved to a separate package.
-To migrate, please install "@react-native/jest-preset" and update your
-jest.config.js to reference:
-  preset: '@react-native/jest-preset'`,
-    );
-  } else {
-    throw error;
-  }
-}
diff --git a/packages/react-native/package.json b/packages/react-native/package.json
index fe57f01f9109..429675458c65 100644
--- a/packages/react-native/package.json
+++ b/packages/react-native/package.json
@@ -52,7 +52,6 @@
       "types": null,
       "default": "./src/asset-registry.js"
     },
-    "./jest-preset": "./jest-preset.js",
     "./rn-get-polyfills": "./rn-get-polyfills.js",
     "./src/fb_internal/*": "./src/fb_internal/*",
     "./package.json": "./package.json"
@@ -69,7 +68,6 @@
     "gradle/libs.versions.toml",
     "index.js",
     "index.js.flow",
-    "jest-preset.js",
     "Libraries",
     "LICENSE",
     "React-Core.podspec",
@@ -136,16 +134,12 @@
     "featureflags": "node ./scripts/featureflags/index.js"
   },
   "peerDependencies": {
-    "@react-native/jest-preset": "0.87.0-main",
     "@types/react": "^19.1.1",
     "react": "^19.2.3"
   },
   "peerDependenciesMeta": {
     "@types/react": {
       "optional": true
-    },
-    "@react-native/jest-preset": {
-      "optional": true
     }
   },
   "dependencies": {