Fiber-seq ns/nl as/al tags

[robert-a-forsyth]

Hi,

I've been trying to visualize nucleosome and methylation sensitive patches predicted by fibertools. It encodes the start site (ns/as for nucleosomes and msp respectively) and the length (nl/al) in the bam tags. I was wondering if there was a way to adjust how IGV interprets the tags for visualizing these?

At the moment I just get each read colored differently, hoping to see if there is someway to visualize just the distance the nucleosome/msp covers or if I need to find a more creative solution to this

[jrobinso]

There's nothing in IGV currently for this. If you can provide some test data I'll have a look at it.

[pontushojer]

Hi, I am also interested in this feature. Here is a link to a public HiFi Fiber-seq BAM with the relevant SAM tags, curtesy of PacBio.

https://downloads.pacbcloud.com/public/revio/2025Q3/WGS/CHM13_Fiber-seq/m84039_250808_044149_s4.hifi_reads.bc2084.chm13v2p0_noY.hp.nuc.fire.bam